| Synthesis and Identification of Artificial Antigen for Imidacloprid |
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| 摘 要： |
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| 关 键 词： | 抗体 人造抗原 土壤 种子 碱性条件 |
Synthesis and Identification of Artificial Antigen for Imidacloprid |
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| Authors: | ZHU Guo-nian GUI Wen-jun ZHENG Zun-tao CHENG Jing-li WU Gang |
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| Institution: | 1. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, P.R. China
2. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310012, P.R. China |
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| Abstract: | This study reported that a hapten of imidacloprid, 1-(6-Carboxylethylthio-3- pyridinyl)methyl)-N-nitro-imidazolidinimine was synthesized using technical material of imidacloprid to react with 3-mercaptopropionic acid in hot alkaline solution. The hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating antigen, respectively. The antibody with high titer (2.56 × 104) was obtained after immuning rabbits. The results showed that the antibody had a high affinity to imidacloprid tested by enzyme-linked immunosorbent assay (ELISA), which IC50 and IC20 were 20.7 and 1.1 μg L−1; and the cross reactibilities to those compounds related with imidacloprid were less than 1.4%. |
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| Keywords: | imidacloprid artificial antigen antibody |
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