| 兔出血症病毒衣壳蛋白VP60在杆状病毒表达系统中的表达及细胞内定位 |
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| 引用本文： | 陈柳,云涛,刘光清,倪征,余斌,宋毅. 兔出血症病毒衣壳蛋白VP60在杆状病毒表达系统中的表达及细胞内定位[J]. 浙江农业学报, 2010, 22(2) |
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| 作者姓名： | 陈柳 云涛 刘光清 倪征 余斌 宋毅 |
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| 作者单位： | 1. 浙江省农业科学院,农业部病毒学与生物技术重点实验室,浙江,杭州,310021
2. 浙江省农业科学院,农业部病毒学与生物技术重点实验室,浙江,杭州,310021;浙江师范大学,化学与生命科学学院,浙江,金华,321004 |
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| 基金项目： | 国家自然科学基金，浙江省农业科学院与中国科学院合作项目，浙江省自然科学基金 |
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| 摘 要： | 为了探讨兔出血症病毒(RHDV)衣壳蛋白的细胞内定位,利用杆状病毒表达系统构建了表达RHDV结构蛋白(VP60)的重组杆状病毒。间接免疫荧光(IFA)和Western blot检测以及电子显微镜观察结果表明,VP60不仅能在昆虫细胞中正确表达,而且能自组装形成病毒样颗粒(VLPs)。进一步对表达蛋白进行细胞内定位观察发现,表达蛋白定位于Sf9细胞的细胞膜上。
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| 关 键 词： | 兔出血症病毒 结构蛋白 昆虫-杆状病毒表达系统 细胞内定位 |
Expression and localization analysis of VP60 protein of Rabbit hemorrhagic disease virus in insect cells-baculovirus expression system |
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| CHEN Liu,YUN Tao,LIU Guang-qing,NI Zheng,YU Bin,SONG Yi. Expression and localization analysis of VP60 protein of Rabbit hemorrhagic disease virus in insect cells-baculovirus expression system[J]. Acta Agriculturae Zhejiangensis, 2010, 22(2) |
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| Authors: | CHEN Liu YUN Tao LIU Guang-qing NI Zheng YU Bin SONG Yi |
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| Affiliation: | CHEN Liu1,YUN Tao1,LIU Guang-qing1,NI Zheng1,YU Bin1,SONG Yi1,2 (1Key Laboratory of Virology & Biotechnology of Agriculture Ministry,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China,2College of Chemistry , Life Sciences,Zhejiang Normal University,Jinhua 321004,China) |
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| Abstract: | A recombinant baculovirus expressing VP60 of Rabbit hemorrhagic disease virus (RHDV) was constructed with bac-to-bac baculovirus expression system to study the expression and intracellular localization of the structural protein VP60 of RHDV in Sf9 cells.The results of indirect immnuofluorescence assay (IFA) and Western blot analysis showed that the proteins were successfully expressed in Sf9 cells.The results of electron micrographs showed that there were some virus-like particles (VLPs) formed in Sf9 cells... |
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| Keywords: | Rabbit hemorrhagic disease virus structural protein insect cells-baculovirus expression system intracellular localization |
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