| 苜蓿中华根瘤菌与鹰嘴豆中慢生根瘤菌原生质体的融合研究 |
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| 引用本文： | 韦革宏,朱铭莪,郭 杰. 苜蓿中华根瘤菌与鹰嘴豆中慢生根瘤菌原生质体的融合研究[J]. 西北农林科技大学学报(自然科学版), 2002, 30(1): 18-22 |
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| 作者姓名： | 韦革宏 朱铭莪 郭 杰 |
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| 作者单位： | 1. 西北农林科技大学,资源环境学院,陕西,杨陵,712100
2. 中国农业大学,生物学院,北京,100094 |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 0 0 0 0 5 ) |
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| 摘 要： | 利用原生质体融合技术 ,以链霉素和青霉素分别作为苜蓿中华根瘤菌 (Sinorhizobium meliloti)10 2 F2 8和鹰嘴豆中慢生根瘤菌 (Mesorhizobium ciceri ) USDA3383的抗药性选择标记 ,成功地获得了 10 2 F2 8和USDA3383的属间融合子。该融合子可分别在双亲寄主植物上结瘤 ,其在细胞形态、大小和蛋白质电泳图谱上与亲本菌株均有所差异。融合子与 10 2 F2 8的 DNA同源性为 90 .8%,而与 U SDA3383的 DNA同源性为 15 .2 %。
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| 关 键 词： | 原生质体融合 苜蓿中华根瘤菌 鹰嘴豆中慢生根瘤菌 |
| 文章编号： | 1000-2782(2002)01-0018-05 |
| 收稿时间： | 2001-03-07 |
| 修稿时间： | 2001-03-07 |
Study on fusion of protoplasts from Sinorhizobium meliloti and Mesorhizobium ciceri |
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| ZHAO Li ming,CHEN Hao. Study on fusion of protoplasts from Sinorhizobium meliloti and Mesorhizobium ciceri[J]. Journal of Northwest A&F University（Natural Science Edition）, 2002, 30(1): 18-22 |
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| Authors: | ZHAO Li ming CHEN Hao |
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| Affiliation: | 2 (1 College of Resources and Environment,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China; 2 College of Biological Sciences,China Agricultural University,Beijin |
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| Abstract: | Streptomycin and penicillin were regarded as the sign of resistance to antibiotics of Sinorhizobium meliloti 102F28 and Mesorhizobium ciceri USDA3383 respectively.Using the protoplast fusion technique,102F28 and USDA3383 were successfully fused.Fusion hybrid can inoculate in the leguminous of parental strains respectively.There were apparent differences between parents and fusion hybrid in cell morphology,and pattern of whole-cell protein.The values of DNA homology between fusion hybrid and 102F28 and USDA3383 were 90.8% and 15.2% respectively. |
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| Keywords: | Protoplast fusion Sinorhizobium meliloti Mesorhizobium ciceri |
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