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油菜对~(35)SO_4~(2-)吸收和种子中硫代萄萄糖苷的累积
引用本文:张勤争,郎献华,奚海福,朱学群,刘银花. 油菜对~(35)SO_4~(2-)吸收和种子中硫代萄萄糖苷的累积[J]. 核农学报, 1991, 5(1): 60-64
作者姓名:张勤争  郎献华  奚海福  朱学群  刘银花
作者单位:浙江农业大学核农所,浙江农业大学核农所,浙江农业大学核农所,杭州农业专科学校,义鸟县义亭区农技站 杭州 310029,杭州 310029,杭州 310029,杭州,义鸟
摘    要:砂培油菜苗能迅速从培养液中吸收~(35)SO_4~(2-),并迅速结合到硫代葡萄糖苷、氨基酸和蛋白质中。标记后,70%甲醇抽提的~(35)S申硫苷-~(35)S的百分含量随时间有规律地下降,而氨基酸中的~(35)S相应地上升。结合成分中~(35)S的相对含量在标记5天后保持恒定。开花后两周,豆荚和籽粒中硫苷-~(35)S的相对含量随时间线性增加,至开花后8周,种子中70%甲醇抽提成分的~(35)S均为硫苷-~(35)S。豆荚和籽粒中,硫苷-~(35)S与干物质含量均随时间线性增加。

关 键 词:硫代葡萄糖苷    吸收    积累    油菜
收稿时间:2009-12-31
修稿时间:2009-12-31

STUDIES ON ABSORPTION, CONVERSION OF SULFATE BY RAPE SEEDLING AND ACCUMULATION OF GLUCOSINOLATE IN RAPE SEED BY USING ~(35)S
Abstract:The seedlings grown in sandy culture absorbed 35SO42-from cultural solution rapidly and the 35S was incoperated into glucosinolates,amino acids and proteins.Percentage of glucosinolates-35S in extractable.35S with 70% methanol declined and those of amino acid-35S rose regularly with time after labelling.The relative content of 35S incoperated into bound constituents as well as extractable components was constant after 5 days of labelling.From two weeks after flowering,the relative content of GS-35S in pods and seeds increased linearly with time until eight weeks after flowering when all 35S extracted with 70% methanol in seeds were in form of glucosinolate.The amount of 35S-GS per pod increased linearly from the first week to the eighth week after flowering.It seems that the accumulation of GS in seeds is related to the accumulation of dry matter in seeds
Keywords:glucosinolate  absorption  accumulation  rape
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