Crystal violet staining of Bordetella bronchiseptica colonies for differentiation of phase-I strains from variant strains in degraded phases. |
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| Authors: | H Ishikawa and Y Isayama |
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| Affiliation: | Hokkaido Research Station, National Institute of Animal Health, Hitsujiganka, Toyohira-ku, Sapporo, Japan. |
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| Abstract: | After 2 days of growth on Brain heart infusion agar (BHIA) at 38 degrees C, phase-I colonies and degraded-phase colonies of Bordetella bronchiseptica could be differentiated by their ability to take up crystal violet (CV). Phase-I colonies in X mode, but not colonies in degraded phases (phases II, III, and rough) bound CV. Phenotypically-altered C-mode colonies (grown at 32 degrees C or lower temperatures) also lacked this ability. CV staining offers an easy method for the recognition of different colony types that appear identical when observed on BHIA. |
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