| 啤酒酵母多糖的分离纯化与初步鉴定 |
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| 引用本文： | 张丽霞,张丽萍,于文革,袁改霞,袁红梅. 啤酒酵母多糖的分离纯化与初步鉴定[J]. 安徽农业科学, 2010, 38(31): 17371-17372,17374. DOI: 10.3969/j.issn.0517-6611.2010.31.006 |
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| 作者姓名： | 张丽霞 张丽萍 于文革 袁改霞 袁红梅 |
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| 作者单位： | 大庆师范学院生命科学系,黑龙江大庆,163712;东北师范大学生命科学学院,吉林长春,130024 |
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| 基金项目： | 黑龙江省教育厅科学技术研究指导项目(12033005) |
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| 摘 要： | [目的]从啤酒废酵母中分离纯化出多糖,并对其组成和性质进行初步鉴定。[方法]用常规方法分离纯化啤酒酵母多糖,并用HPLC、比旋度测定和醋酸纤维薄膜电泳等方法测定BPS2。[结果]粗多糖收率为9．7％,为浅色粉灰。BPS2为均一组分,苯酚-硫酸法测定其糖含量为95．2％,GC分析其单糖组成为Man和Glc,摩尔比为1．08：1．00。[结论]试验提取的粗多糖为一种新多糖,其主要成分为BPS2,为啤酒酵母的利用提供了基础。
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| 关 键 词： | 啤酒酵母 多糖 分离纯化 组成鉴定 |
Isolation Purification and Ideitification of Polysaccharide Extracted from Beer Yeast |
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| ZHANG Li-xia et al. Isolation Purification and Ideitification of Polysaccharide Extracted from Beer Yeast[J]. Journal of Anhui Agricultural Sciences, 2010, 38(31): 17371-17372,17374. DOI: 10.3969/j.issn.0517-6611.2010.31.006 |
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| Authors: | ZHANG Li-xia et al |
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| Affiliation: | ZHANG Li-xia et al(Department of Life Science,Daqing Normal College,Daqing,Heilongjiang 163712) |
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| Abstract: | [Objective] To extract and purify new polysaccharide from waste beer yeast,and study the composition and characterization of it.[Methods] The waste beer yeast was deproteinized by dissolving cleaning,freezing and thawing,ultrasonic disruption,enzymatic removal of protein combined with the Sevag method,fractionated by ultrafiltration and gel chromatography on CL-6B column,so BPS2 was obtained.Its homogeneity was proved with HPLC,specific optical rotation measurement and cellulose acetate membrane electrophor... |
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| Keywords: | Waste beer yeast Polysaccharide Isolation Composition |
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