| 叶绿素降解菌的筛选与应用 |
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| 引用本文： | 陈志良,李海云,李子院,郝再彬. 叶绿素降解菌的筛选与应用[J]. 湖北农业科学, 2012, 51(14): 2992-2995 |
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| 作者姓名： | 陈志良 李海云 李子院 郝再彬 |
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| 作者单位： | 桂林理工大学化学与生物工程学院,广西桂林,541004 |
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| 基金项目： | 国家自然科学基金项目(31060193);桂林理工大学科研项目(0842003-35) |
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| 摘 要： | 以叶绿素为培养基成分,在不添加无机盐的条件下,从土壤中分离筛选得到一株能降解叶绿素的菌株；经过形态学鉴定,确定它为曲霉属中的一种,菌株命名为GLHZB319,保藏在中国典型培养物保藏中心内,保藏编号为CCTCC No:M2011101.在温度为30℃、摇床转速100 r/min、自然pH、GLHZB319菌株接种孢子量为1.0×106个/mL的培养条件下,把100 mL的叶绿素水溶液培养3d,溶液的吸光度值由0.310降解到0.002,降解率达到99％.
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| 关 键 词： | 叶绿素 降解菌 筛选 |
Screening and Application of Chlorophyll Degradation Bacterium |
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| CHEN Zhi-liang , LI Hai-yun , LI Zi-yuan , HAO Zai-bin. Screening and Application of Chlorophyll Degradation Bacterium[J]. Hubei Agricultural Sciences, 2012, 51(14): 2992-2995 |
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| Authors: | CHEN Zhi-liang LI Hai-yun LI Zi-yuan HAO Zai-bin |
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| Affiliation: | (Chemical and Biological Engineering College,Guilin University of Technology,Guilin 541004,Guangxi,China) |
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| Abstract: | Taking chlorophyll as culture medium component,and adding no inorganic,a strain capable of degrading chlorophyll was isolated from soil.According to morphological identification,it belonged to the genus Aspergillus Mich.ex Link:Fr..The strain was named GLHZB319,and preserved in Chinese Center for Type Culture Collection(Collects number: CCTCC No: M2011101).At 30 ℃,shaking speed 100 r/min,natural pH,with GLHZB319 natural strains inoculation quantity 1.0×106 ind./mL,the absorbance value of 100 mL chlorophyll solution was decreased from 0.310 to 0.002 after 3 d,with a degradation rate of 99%. |
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| Keywords: | chlorophyll decomposition bacterium screening |
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