| 地黄中梓醇的大孔吸附树脂纯化工艺 |
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| 引用本文： | 洪冲,付香斌,刘红记,时延光,王明道.地黄中梓醇的大孔吸附树脂纯化工艺[J].湖北农业科学,2012,51(14):3062-3064,3083. |
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| 作者姓名： | 洪冲 付香斌 刘红记 时延光 王明道 |
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| 作者单位： | 1. 河南农业大学生命科学学院,郑州,450002
2. 河南省轻工业学校,郑州,450006 |
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| 基金项目： | 国家自然科学基金项目(30671201);河南农业大学博士基金项目(30600097);河南农业大学创新基金项目(2009-CX-017) |
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| 摘 要： | 采用优选大孔吸附树脂纯化梓醇,以分光光度法测定梓醇浓度,对纯化地黄中梓醇的大孔吸附树脂工艺进行了研究.结果表明,H103树脂吸附与纯化梓醇的效果较好,上样液浓度为4.08 mg/mL、上样液体积为7个柱体积、洗脱剂为体积分数10％的乙醇、洗脱剂体积为8个柱体积、静态吸附时间为4h为纯化的最优条件.
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| 关 键 词： | 大孔吸附树脂 地黄 梓醇 纯化 |
Separation and Purification of Catalpol from Radix Rehmanniae by Macroporous Adsorption Resins |
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| HONG Chong
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FU Xiang-bin
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LIU Hong-ji
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SHI Yan-guang
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WANG Ming-dao.Separation and Purification of Catalpol from Radix Rehmanniae by Macroporous Adsorption Resins[J].Hubei Agricultural Sciences,2012,51(14):3062-3064,3083. |
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| Authors: | HONG Chong FU Xiang-bin LIU Hong-ji SHI Yan-guang WANG Ming-dao |
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| Institution: | 1(1.Life Science Institute,Henan Agricultural University,Zhengzhou 450002,China; 2.Henan School for Light Industry,Zhengzhou 450006,China) |
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| Abstract: | Catalpol was extracted from Radix Rehmanniae by selecting propriate macroporous adsorption resins and determined by spectrophotometry method.The results showed that H103 was the best resins for catalpol adsorption and purification.The optimal conditions were,concentration of loading sample,4.08 mg/mL;sampling volume,7 BV;eluent,10% ethanol;elution volume,8 BV,static obsorption time,4 h. |
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| Keywords: | macroporous adsorption resin Radix Rehmanniae catapol purification |
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