三种寡核苷酸纯化方法的比较

分别采用固相萃取法、电泳法、色谱法纯化寡核苷酸,色谱分析结果表明产物纯度高,固相萃取法纯化后的产物纯度低于电泳法与色谱法,但产量相对较大,PCR检测结果证明3种方法纯化的寡核苷酸具有良好的检测性能.在建立的固相萃取纯化试验条件下,对3种填料纯化寡核苷酸的效果、产量、成本进行了比较分析,结果表明,使用硅胶C绣和聚苯乙烯-二乙烯基苯填料(PS/DVB)填充的萃取小柱既能达到良好的纯化效果又可降低成本.

Comparison of Three Purification Methods for Oligonucleotides

Solid-phase extraction method,electrophoresis method and chromatography method were applied for purifying crude oligonucleotides.The results of chromatography analysis showed that the product was with high purity.The purity of product from solid-phase extraction method was a little bit lower than that of electrophoresis method;but the yield was relatively higher.According to PCR test,all the 3 methods has good detection performance.Under the solid phase extraction purification test conditions,the purification effect,yield and cost of three kinds of fillers was compared.The result showed that good purification effect with low cost could be obtained when using silica gel C18 and PS/DVB filling extraction columns.