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百合多糖提取及成分分析
引用本文:张国强,杨正平.百合多糖提取及成分分析[J].湖北农业科学,2012,51(15):3298-3299.
作者姓名:张国强  杨正平
作者单位:安阳工学院生物与食品工程学院,河南安阳,455000
摘    要:采用水提醇沉法提取百合(Bulbus lilii)中的多糖成分,Sevage法除蛋白质,凝胶色谱法进行多糖纯化,制备3-甲基-1-苯基-2-吡唑啉酮(PMP)衍生物进行高效液相色谱分析.结果表明,百合多糖由鼠李糖、阿拉伯糖、葡萄糖、木糖、半乳糖5种单糖组成,其含量分别为4.82%、12.79%、47.02%、13.01%、22.36%.

关 键 词:百合(Bulbus  lilii)  多糖  提取  成分分析

Extraction and Composition Analysis of Polysaccharides in Bulbus lilii
ZHANG Guo-qiang , YANG Zheng-ping.Extraction and Composition Analysis of Polysaccharides in Bulbus lilii[J].Hubei Agricultural Sciences,2012,51(15):3298-3299.
Authors:ZHANG Guo-qiang  YANG Zheng-ping
Institution:(School of Biotechnology and Food,Anyang Institute of Technology,Anyang 455000,Henan,China)
Abstract:Polysaccharide was extracted and isolated from Bulbus lilii by water extracting-alcohol precipitating method and purified by Sevage method and gel permeation chromatography.The components of the polysaccharide were identified and quantified by HPLC of the PMP derivatives.The results showed that the main monosaccharides contained in B.lilii polysaccharide were rhamnose,arabinose,glucose,xylose and galactose,with content of 4.82%,12.79%,47.02%,13.01% and 22.36%,respectively.
Keywords:Bulbus lilii  polysaccharide  extraction  component analysis
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