磨盘柿的合子胚挽救培养

 以‘西村早生’为父本与‘磨盘柿’杂交, 对杂交后的磨盘柿合子胚进行了挽救培养。利用正交试验法研究了改良MS培养基中N、蔗糖、6-BA和IBA浓度4个因素及其组合对磨盘柿合子胚萌发、生根及成苗的综合影响。结果表明: (1) 蔗糖浓度对合子胚的萌发生长影响最显著, 6-BA和IBA次之, N浓度仅对叶色的影响较大; (2) 最佳的培养基以(3 /4N) MS为基本培养基, 添加6-BA 0.1 mg·L - 1和IBA 0.02 mg·L ^{- 1} , 蔗糖最适浓度为50 g·L ^-1。

In Vitro Rescue Culture of Zygotic Embryo of‘Mopanshi’Persimmon

The zygotic embryos, obtained from crossing between Chinese persimmon ‘Mopanshi’ (Diospyros kaki Thunb. ‘Mopanshi’) and Japanese cultivar‘Nishimura Wase’, were rescued under in vitro conditions. The effect of nitrogen (N) concentration in MS medium, and the effects of sucrose, 6-BA andIBA concentrations on the rates of sprouting and lateral rooting and plantlet formation were investigated using Orthogonal design at 3 levels. The results showed that sucrose concentration was the most effective factor, then, followed by IBA and 6-BA in these four factors. The concentrations of N in MS medium exhibited no considerable effect to rates of sprouting and lateral rooting and plantlet formation, but showing prominent effect to leaf color. This study also demonstrated that the optimum medium for embryo rescue was MS basal medium containing 3 /4N + 6-BA 0.1 mg·L ^{- 1} + IBA 0.02 mg·L ^{- 1} + sucrose 50 g·L ^{- 1}.