| 山葡萄基因组DNA提取及RAPD鉴定 |
| |
| 引用本文： | 王军,贺普超.山葡萄基因组DNA提取及RAPD鉴定[J].果树学报,2000,17(2):79-82. |
| |
| 作者姓名： | 王军 贺普超 |
| |
| 作者单位： | 1. 中国农业科学院特产研究所,吉林,132109
2. 西北农业大学园艺系 |
| |
| 摘 要： | 以雌花山葡萄（７３０６４）冷藏幼叶、香妃葡萄新鲜幼叶为试材，分别采用修改的ＣＴＡＢ法、高盐法、ＳＤＳ法提取基因组ＤＮＡ。结果表明，三种方法所提的ＤＮＡ纯度及产率有差别，从综合结果看，以ＣＴＡＢ法为好，所得ＤＮＡ不需经氯化艳梯度离心或柱层析，可直接用于ＲＡＰＤ分析。
|
| 关 键 词： | 山葡萄 基因组DNA提取 RAPD |
Extraction of Genomic DNA from Vitis amurensis and Its RAPD Analysis |
| |
| Wang Jun,He Puchao.Extraction of Genomic DNA from Vitis amurensis and Its RAPD Analysis[J].Journal of Fruit Science,2000,17(2):79-82. |
| |
| Authors: | Wang Jun He Puchao |
| |
| Abstract: | The extracts of high quality genomic DNA was extracted from the frozen leaves of V. amurensis Rupr. (■,73064) and the fresh leaves of Xiangfei(V. vinifera L. )by modified CTAB, medium with high salts and SDS method respectively. The purity and production were different among those 3 methods. According to the comprehensive results, the method of GB was the best (A260/A280 = 1. 7, production 57 ng' mg~(-1) fresh leaves). ms method didn' t need the liquid N and phenol extraction, and the DNA length was similar to λ DNA (48 kb) .TIs isolated DNA could be used directly for RAPD analysis without sedimentation in cesium chloride gradient or column chromatography. It's really suitable for great amount samples for RAPD analysis. |
| |
| Keywords: | V amurensis Extraction of Genomic DNA RAPD |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
