| 放线菌Z139菌株的分离、鉴定及其生物活性 |
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| 引用本文： | 张波,吴文君,宗兆锋.放线菌Z139菌株的分离、鉴定及其生物活性[J].西北农林科技大学学报(社会科学版),2005,33(8):69-72. |
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| 作者姓名： | 张波 吴文君 宗兆锋 |
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| 作者单位： | 1. 陕西省出入境检验检疫局,陕西,西安,710068
2. 西北农林科技大学,植保学院,陕西,杨凌,712100 |
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| 基金项目： | 国家863高技术计划项目（2002AA245121） |
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| 摘 要： | 从陕西秦岭山区采集的土样中分离到1株具有广谱抗真菌活性的放线菌菌株Z139,经初步鉴定为小多孢菌属红棕褐小多孢菌(Micropolysporarabrobranea)的一个变种。该菌株发酵液对玉米小斑病菌、苹果轮纹病菌、棉花枯萎病菌、马铃薯干腐病菌和西瓜枯萎病菌菌丝的生长抑制中浓度(EC50)分别为0.979,0.505,1.733,0.425和0.849mL/L,对玉米大斑病菌、番茄叶霉病菌、马铃薯干腐病菌和烟草赤星病菌的孢子萌发抑制中浓度(EC50)分别为1.709,0.375,0.445和1.333mL/L。对小麦白粉病的保护和治疗作用分别达到98.6%和86.1%。
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| 关 键 词： | 放线菌 杀菌活性 红棕褐小多孢菌 |
| 文章编号： | 1671-9387（2005）08-0069-04 |
| 收稿时间： | 2004-11-11 |
| 修稿时间： | 2004年11月11 |
Studies on isolation,identification and fungicidal activity of antagonistic actinomycete Z139 |
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| ZHANG Bo,WU Wen-jun,ZONG Zhao-feng.Studies on isolation,identification and fungicidal activity of antagonistic actinomycete Z139[J].Journal of Northwest Sci-Tech Univ of Agr and,2005,33(8):69-72. |
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| Authors: | ZHANG Bo WU Wen-jun ZONG Zhao-feng |
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| Institution: | 1 Shaanxi Entry-Exit Inspection and Quarantine Burea,Xi’an,Shaanxi 710068,China;2 College of Plant Protection,Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | A Actinomycete strain producing a broad-spectrum antifungal substance was isolated from the soil of the Qinling mountain in Shaanxi. The strain numbered Z139 was initially identified as a variety of Micropolyspora rabrobranea. The results of mycelium growth rate showed that the value of EC50 against Bipolaria maydis,Macrophoma kawatsukai, Fusarium oxysporum, Fusarium solani and Fusarium oxysporium were 0. 979,0. 505,1. 733,0. 425 and 0. 849 mL/L. The result of spore germination proved that the value of EC50 against Alternaria longipes, Ecrserohilum trucicum, Fulvia fulva and Fusarium solani were 1. 709,0. 375,0. 445 and 1. 333 mL/L. The results of pot test indicated that fermention products of Z139 exhibited 98.6% of protected efficacy and 86.1% of therapeutic efficacy. |
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| Keywords: | actinomycetes fungicidal activity Micropolyspora rabrobranea |
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