| 山东省禽源致病性大肠杆菌质粒介导喹诺酮类药物耐药基因的检测 |
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| 引用本文： | 苏晶,杨芳芳,孙慧,朱岩丽,王淑静,谢之景,毕海洋,姜世金. 山东省禽源致病性大肠杆菌质粒介导喹诺酮类药物耐药基因的检测[J]. 山东农业科学, 2012, 44(9): 5-8 |
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| 作者姓名： | 苏晶 杨芳芳 孙慧 朱岩丽 王淑静 谢之景 毕海洋 姜世金 |
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| 作者单位： | 1. 山东农业大学动物医学院/山东省动物生物工程与疾病防治重点实验室,山东泰安,271018
2. 淄博市博山区畜牧局,山东淄博,255200 |
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| 基金项目： | 山东省高等学校科技计划项目，山东省科技发展计划项目 |
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| 摘 要： | 为了解山东省禽源致病性大肠杆菌质粒介导喹诺酮类药物耐药基因分布情况,对2004～2011年间分离的230株禽源致病性大肠杆菌进行了qnrA、qnrB、qnrS基因的PCR检测,结果发现未检出qnrA和qnrB,qnrS基因的检出率为25.22%(58/230)。对部分检出的qnrS基因测序后发现假阳性率较高(8/24),为了提高检测的特异性,建立了检测qnrS基因的套式PCR,测序结果表明该方法检出准确率为100%,使用该方法检出230株细菌中qnrS基因的携带率为16.52%(38/230),表明含qnrS基因的耐药质粒在山东省禽源致病性大肠杆菌中分布较为广泛。
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| 关 键 词： | 大肠杆菌 喹诺酮类药物 耐药性 qnrS基因 套式PCR |
Detection of Quinolone Resistance Genes in Plasmid among Avian Escherichia coli Strains from Shandong Province |
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| SU Jing , YANG Fang-fang , SUN Hui , ZHU Yan-li , WANG Shu-jing , XIE Zhi-jing , BI Hai-yang , JIANG Shi-jin. Detection of Quinolone Resistance Genes in Plasmid among Avian Escherichia coli Strains from Shandong Province[J]. Shandong Agricultural Sciences, 2012, 44(9): 5-8 |
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| Authors: | SU Jing YANG Fang-fang SUN Hui ZHU Yan-li WANG Shu-jing XIE Zhi-jing BI Hai-yang JIANG Shi-jin |
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| Affiliation: | 1*(1.College of Veterinary Medicine,Shandong Agricultural University/ Shandong Key Laboratory of Animal Biotechnology and Disease Control and Prevention,Taian 271018,China;2.Boshan Animal Husbandry Bureau,Zibo 255200,China) |
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| Abstract: | In order to study the prevalent of resistance genes in plasmid to quinolone among avian Escherichia coli strains from Shandong Province,a total of 230 strains isolated in 2004~2011 were tested by PCR to examine the qnrA,qnrB and qnrS genes which mediated quinolone resistance.The results indicated that the present ratio of qnrA,qnrB and qnrS genes were 0,0 and 25.22%(58/230),and there was high false positive rate in detection of qnrS gene(8/24) by sequencing some PCR positive samples.In order to improve the detection specificity of qnrS gene,a nested-PCR method was established and used to detect the 230 E.coli strains.The sequencing results indicated that the accuracy of the method was 100%,and the present ratio of qnrS gene was 16.52%(38/230),which showed that the plasmid with quinolone resistance gene qnrS was more widely distributed in avian Escherichia coli strains from Shandong Province. |
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| Keywords: | Escherichia coli Quinolone Resistance qnrS gene Nested PCR |
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