| TERF1基因增强糖橙抗病性的初步研究 |
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| 引用本文： | 罗赛男,邓子牛,易图永,黄荣峰,曾柏全. TERF1基因增强糖橙抗病性的初步研究[J]. 中南林业科技大学学报(自然科学版), 2008, 28(5) |
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| 作者姓名： | 罗赛男 邓子牛 易图永 黄荣峰 曾柏全 |
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| 作者单位： | 湖南农业大学园艺园林学院，国家柑橘改良中心长沙分中心，湖南农业大学生物安全技术学院，湖南省农业科学院园艺研究所，中国农业科学院生物技术研究所，中南林业科技大学生命科学与技术学院 |
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| 基金项目： | 国家柑橘转基因专项基金 |
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| 摘 要： | 将获得转TERF1基因糖橙的春梢叶片进行柑桔溃疡病和炭疽病的接种.初步试验表明,TERF1基因增强了糖橙的广谱抗病性.在接种溃疡病后,转基因植株的平均发病率下降了50%左右,且发病的时间比冰糖橙和对照推迟了7 d,其中3号株系的抗病性最强,叶片无病症.柑橘叶片接种炭疽病后,转基因植株的叶片与对照相比基本上表现出针孔处病斑减小,延迟发病等现象.其中3号和11号转TERF1基因糖橙叶片上根本观察不到炭疽病的典型病斑,且叶片也无褪绿.
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| 关 键 词： | 生物技术 果树育种 糖橙 TERF1基因 柑橘溃疡病 柑橘炭疽病 |
Preliminary Results of Succari's Disease Resistance Enhanced with TERF1 Gene |
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| LUO Sai-nan,DENG Zi-niu,ZHONG Xiao-hong,YI Tu-yong,HUANG Rong-feng,ZENG Bai-quan. Preliminary Results of Succari's Disease Resistance Enhanced with TERF1 Gene[J]. , 2008, 28(5) |
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| Authors: | LUO Sai-nan DENG Zi-niu ZHONG Xiao-hong YI Tu-yong HUANG Rong-feng ZENG Bai-quan |
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| Abstract: | An experiment was conducted by inoculating citrus bacterial canker and citrus anthracnose to the leaves of transgenic Succari.Results show that TERF1 gene can enhance the transgenic plants with their broad-spectrum disease resistance; that after an inoculation with citrus bacterial canker,their incidence rate is reduced by 50%;that compared to Bingtang Orange and CK,transgenic Succari also delays the expression of disease symptoms in 7 days and the inoculated plant No.3 has displayed no symptoms at all;that after an inoculation with citrus anthracnose,the contrast plants display typical symptoms in 7 days but the transgenic plants show a size reduction of diseased spots and a delay of diseased time;and that the inoculated plants No.3 and No.11 even show evident resistance to citrus anthracnose. |
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| Keywords: | biotechnology fruit breeding Succari TERF1 gene citrus bacterial canker citrus anthracnose |
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