Detection of Grapevine A vitivirus in Tunisian grapevines |
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| Authors: | S. M'hirsi S. Fattouch H. Acheche M. Marrakchi N. Marzouki |
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| Affiliation: | Laboratoire de Génie Biologique, Institut National des Sciences Appliquées et de Technologie, INSAT, Centre Urbain Nord, BP no. 676, Tunis 1080 (Tunisie);e-mail:;Laboratoire de Génétique et Biologie Moléculaire, Facultédes Sciences de Tunis, Campus Universitaire 1002 (Tunisie) |
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| Abstract: | Grapevine A vitivirus (GVA), Grapevine B vitivirus (GVB), Grapevine leaf-roll associated closterovirus 3 (GLRaV3) and Grapevine fanleaf nepovirus (GFLV) were detected by DAS-ELISA in samples of grapevine showing symptoms of rugose wood from different viticultural areas of Tunisia. After optimization of experimental conditions, PCR and IC-RT–PCR were found to be more efficient and sensitive than DAS-ELISA for the detection of GVA. |
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