| 几个桃优质新品种的RAPD鉴定与分析 |
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| 引用本文： | 李莉,刘玉祥,彭建营.几个桃优质新品种的RAPD鉴定与分析[J].河北农业大学学报,2010,33(1). |
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| 作者姓名： | 李莉 刘玉祥 彭建营 |
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| 作者单位： | 1. 河北农业大学园艺学院,河北,保定,071001
2. 唐山职业技术学院,河北,唐山,063004 |
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| 基金项目： | 国家自然科学基金资助项目(30270927);;河北省自然科学基金项目(C2004000363);;国家农业科技成果转化资金项目(2007GB2A200034) |
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| 摘 要： | 以6个桃品种为研究对象,采用RAPD技术对其进行鉴定和亲缘关系分析。25个随机引物共获得213条DNA谱带,其中65条(30.5%)为多态性带。通过单引物和引物组合可以将6个品种区分开来。RAPD分析结果表明:"秋红晚蜜"和"秋红早蜜"先聚在一起,然后再与"秋红蜜"聚在一起,三者间遗传相似系数为0.983~0.984,为共同起源的地方性品种;"燕红"和"西妃"先聚在一起,然后又与"乐林早红"聚在一起,三者相似系数范围为0.913~0.980,其中"燕红"和"西妃"的相似系数为0.980。推测"西妃"是"燕红"的芽变或者亲本来源相同。
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| 关 键 词： | 桃 RAPD 品种鉴定 亲缘关系 |
Indentification and analysis on several new peach cultivars by RAPD markers |
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| Ll Li,LIU Yu-xiang,PENG Jian-ying.Indentification and analysis on several new peach cultivars by RAPD markers[J].Journal of Agricultural University of Hebei,2010,33(1). |
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| Authors: | Ll Li LIU Yu-xiang PENG Jian-ying |
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| Institution: | 1.College of Horticulture;Agricultural University of Hebei;Baoding 071001;China;2.Tangshan Vocational Technical College;Tangshan 063004;China |
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| Abstract: | In this study,6 peach cultivars were analyzed using random amplified polymorphic DNA markers.213 DNA bands were amplified with 25 primers,65 of which(30.5%) were polymorphic.Six cultivars can be differentiated through single primer and double primers.The result of cluster analysis showed that 'Qiuhongwanmi' and 'Qiuhongzaomi' were clustered first,and then clustered with 'Qiuhongmi',and the genetic similarity coefficients of the three cultivars ranged from 0.983 to 0.984;They were local cultivars originated ... |
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| Keywords: | peach RAPD cultivars identification phylogenetic relationship |
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