低蛋白天然胶乳的制备及其性能研究

应用2709碱性蛋白酶与稳定体系并用脱除天然胶乳（NRL）蛋白质的生物化学方法,采用多变元析因设计安排试验,研究了低蛋白天然胶乳（LPNRL）的稳定体系、酶的用量及制备条件、LPNRL的贮存及应用性能。研究结果表明:（1）2709碱性蛋白酶与稳定体系并用对鲜胶乳的脱蛋白效果较好;（2）所制备的LPNRL的氮含量为0.06％～0.07％,水溶性蛋白质（WSP）含量为1.8μg/g胶膜,达到国际上同类型产品的先进水平;（3）与正常NRL相比,LPNRL的硫化速率稍慢,但其硫化胶乳的贮存性能稳定,成膜性能良好;其干胶膜的物理性能稍低,但差别不大。 

Preparation of Low Protein Natural Rubber Latex and Its Properties

The stabilizing system, the amount of enzyme and the conditions for preparation of low protein natural rubber latex (LPNRL) as well as its properties in storage and application were studied by use of a biochemical method in which the basic protease 2709 was applied in combination with the stabilizing system in order to remove the protein in natural rubber latex. The experiment was arranged with a multivariate factorial design. The results showed better deproteinization of fresh rubber latex when the basic protease 2 709 was used in combination with the stabilizing system. The nitrogen content in the LPNRL prepared was 0.06 %0.07 % and the content of water soluble protein in the rubber film was 1.8 g/g, falling within the advanced world standards for this type of product.The LPNRL, as compared with the normal NRL, had slightly low vulcanization rate, a stable storage property and a satisfactory film forming property. The dry rubber film had a slightly poor physicalproperty but without significant difference.