| O型和Asia1型口蹄疫病毒感染RT-PCR鉴别诊断方法的建立 |
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| 引用本文： | 涂亚斌,周国辉,张亚科,王志国,张守红,王海伟,于力. O型和Asia1型口蹄疫病毒感染RT-PCR鉴别诊断方法的建立[J]. 中国预防兽医学报, 2007, 29(7): 561-564,568 |
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| 作者姓名： | 涂亚斌 周国辉 张亚科 王志国 张守红 王海伟 于力 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 根据GenBank中O型和Asia1型口蹄疫病毒(Foot-and-mouth disease virus,FMDV)的vp3、vp1和2A基因序列,并与其它血清型FMDV的对应基因序列进行比较,设计用于扩增O型和Asia1型FMDV vp1基因的特异性引物,建立O型和Asia1型FMDV RT-PCR鉴别诊断方法。本方法首先用通用型引物进行RT-PCR,确定是否为FMDV感染,然后用特异性引物鉴别O型或Asia1型FMDV的感染。用vp1基因序列分析进行符合性试验,验证了该方法所具有的特异性和敏感性。本方法可用于O型和Asia1型FMD的快速诊断及流行病学调查。
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| 关 键 词： | O型口蹄疫病毒 Asia1型口蹄疫病毒 鉴别诊断方法 |
| 文章编号： | 1008-0589（2007）07-0561-04 |
| 修稿时间： | 2006-12-05 |
Establishment of a RT-PCR method for differential diagnosis of foot-and-mouth disease virus infection by O and Asia1 serotypes |
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| TU Ya-bin,ZHOU Guo-hui,ZHANG Ya-ke,WANG Zhi-guo,ZHANG Shou-hong,WANG Hai-wei,YU Li. Establishment of a RT-PCR method for differential diagnosis of foot-and-mouth disease virus infection by O and Asia1 serotypes[J]. Chinese Journal of Preventive Veterinary Medicine, 2007, 29(7): 561-564,568 |
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| Authors: | TU Ya-bin ZHOU Guo-hui ZHANG Ya-ke WANG Zhi-guo ZHANG Shou-hong WANG Hai-wei YU Li |
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| Affiliation: | Division of Livestock Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China |
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| Abstract: | A RT-PCR method was developed to differentiate O and Asia1 type foot-and-mouth disease virus(FMDV) infections using primers specific to 1C,1D and 2A genes of FMDV Asia1,O and other serotypes,respectively.FMDV infection was first identified using universal primers,followed by using specific primers to differentiate O and Asia1 serotypes.The specificity and sensitivity of the method was tested and confirmed in FMDV infected cells and by sequence analysis of VP1.This method could be used for rapid diagnosis and epidemiology survey of FMD. |
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| Keywords: | RT-PCR |
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