表达 HarpinEa基因的转基因马铃薯的晚疫病抗性分析

 通过根癌农杆菌介导,用Harpin_Ea基因转化马铃薯四倍体栽培种大西洋（Atlantic）,获得107个马铃薯转化株系。经过卡那霉素的抗性鉴定和PCR分子检测,有59株PCR检测成阳性,占所有转化植株的55.14%.实验对部分转基因植株进行室内抗病性评价,从24个PCR阳性株系中筛选到9个株系的抗病性与对照相比有显著差异(P<0.05),其中7株的抗病性与对照相比差异极显著(P<0.001)。结果初步表明Harpin_Ea基因已整合到马铃薯的基因组中并得到表达,提高了植株的晚疫病抗性。

Analysis of Late Blight Resistance in Transgenic Potato Expressing HarpinEa Gene

A potato commercial cultivar Atlantic was transformed with Harpin Ea gene via Agrobacterium tumefaciens -mediated gene transfer. One hundred and seven regenerated potato plants were obtained. Kanamycin resistance and PCR amplification analysis showed that 59 putative transgenic plants have the Harpin Ea gene,the transformation ratio was 55.14 perpcent. The late blight resistance of some putative transgenic plants was tested in the laboratory. Nine plants in 24 transgenic plants had significant resistance at the level of P <0.05, and 7 plants had high resistance at the level of P <0.001 compared to control in the laboratory. Tests showed that the transformation of Harpin Ea gene improved potato resistance to late blight.