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印水型细胞质雄性不育粳稻恢复系分子标记辅助选择
引用本文:王先俱,谭学林,邵国军,马慧,陈亚君,张城,商文奇.印水型细胞质雄性不育粳稻恢复系分子标记辅助选择[J].分子植物育种,2010,8(2):319-324.
作者姓名:王先俱  谭学林  邵国军  马慧  陈亚君  张城  商文奇
作者单位:1. 云南农业大学稻作研究所,昆明,650201;辽宁省稻作研究所,沈阳,110101
2. 云南农业大学稻作研究所,昆明,650201
3. 辽宁省稻作研究所,沈阳,110101
4. 沈阳农业大学,沈阳,110161
基金项目:辽宁省科技攻关项目,辽宁省科技基金 
摘    要:本文以广亲和材料晚轮422,野败型恢复系蜀恢527、密阳46、密阳42为亲本,在杂交组合的F2、F4中,采用分子标记RM10353、RM6100辅助选择,结合常规育种方法,选育印水型细胞质雄性不育粳稻恢复系。研究表明,经过分子标记检测,同时含有两个恢复基因的水稻植株,测配子一代的结实率明显提高、杂种优势显著。结果表明基于分子标记辅助选育印水型恢复系是可行的。

关 键 词:印水型细胞质雄性不育系  粳稻恢复系  分子标记辅助选择

Molecular Marker-assisted Selection of Japonica Rice Restorer Line for Yinshui Type CMS
Wang Xianju,Tan Xuelin,Shao Guojun,Ma Hui,Chen Yajun,Zhang Cheng,Shang Wenqi.Molecular Marker-assisted Selection of Japonica Rice Restorer Line for Yinshui Type CMS[J].Molecular Plant Breeding,2010,8(2):319-324.
Authors:Wang Xianju  Tan Xuelin  Shao Guojun  Ma Hui  Chen Yajun  Zhang Cheng  Shang Wenqi
Abstract:In this paper,we employed the rice variety Wanlun422 with wide compatibility,WA-type restorer line Shuhui527,Miyang46,and Miyang42 to be as parent materials,and selected the japonica rice restorers line for Yinshui type cytoplasmic male sterility (YS-CMS) from the hybrid combinations F2 and F4 using molecular markerassisted selection combined with the conventional breeding method.The result showed that the plants containing two restorer genes,which were identified by molecular markers,were selected to make hybrid combinations with YS-CMS lines,we found that the seed-setting rate and heterosis of the hybrids were significantly improved.The results indicate that it is feasible for development of restorers for Japonica YS-CMS line based on molecular markerassisted.
Keywords:Yinshui type cytoplasmic male sterility (YS-CMS)  Japonica restorer line  Molecular marker-assisted selection
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