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Analysis of Dynamic Accumulation of Three Types of Glutenin Subunits and Their Content in Relation to Sedimentation Value in Common Wheat
作者姓名:HAO  Jun-jie  CHEN  Jun-ying  CHEN  Xin-jian  CUI  Dang-qun  CHENG  Xi-yong
作者单位:[1]College of Agronomy, Henan Agricultural University, Zhengzhou 450002, P.R.China [2]Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China
基金项目:The authors acknowledge the financial support from the Chinese National Special Foundation for Transgenic Plant Research and Commercialization (TY03-B-19-2) and the Henan Province 0utstanding Individual Innovation Foundation (022100090). In addition, we thank Dr. Joy Fleming for editing the language in this paper.
摘    要:The biosynthetic time and accumulations of A-, B-, and C-type glutenin subunits in 7 winter wheat cultivars with different quality (strong, medium, weak gluten) were analyzed by SDS-PAGE. The results showed that no glutenin subunit was observed within 8 d after anthesis. Parts or all A-, B-, and C-type subunits appeared around day 12 in different cultivars. Other A-, B-, and C-type subunits appeared gradually. The accumulation of A-, B-, and C-type subunits fluctuated before maturity. The results of analysis of correlation between the ratios of A/T (total content of glutenin subunits), A/C, AJ (B+C), (A+B)/C, and (A+B)/T and SDS-sedimentation value suggested that they were more significant. The negative correlation between the ratio of (B+C)/T and SDS-sedimentation value was more significant, and the correlations between the ratio C/T and the SDS-sedimentation value were significantly negative.

关 键 词:麦谷蛋白  小麦  亚单元  堆积作用  品质
收稿时间:16 May 2005

Analysis of Dynamic Accumulation of Three Types of Glutenin Subunits and Their Content in Relation to Sedimentation Value in Common Wheat
HAO Jun-jie CHEN Jun-ying CHEN Xin-jian CUI Dang-qun CHENG Xi-yong.Analysis of Dynamic Accumulation of Three Types of Glutenin Subunits and Their Content in Relation to Sedimentation Value in Common Wheat[J].Agricultural Sciences in China,2006,5(2):155-160.
Authors:HAO Jun-jie  CHEN Jun-ying  CHEN Xin-jian  CUI Dang-qun  CHENG Xi-yong
Institution:1. College of Agronomy, Henan Agricultural University, Zhengzhou 450002, P.R. China;Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China
2. College of Agronomy, Henan Agricultural University, Zhengzhou 450002, P.R. China
Abstract:The biosynthetic time and accumulations of A-, B-, and C-type glutenin subunits in 7 winter wheat cultivars with different quality (strong, medium, weak gluten) were analyzed by SDS-PAGE. The results showed that no glutenin subunit was observed within 8 d after anthesis. Parts or all A-, B-, and C-type subunits appeared around day 12 in different cultivars. Other A-, B-, and C-type subunits appeared gradually. The accumulation of A-, B-, and C-type subunits fluctuated before maturity. The results of analysis of correlation between the ratios of A/T (total content of glutenin subunits), A/C, A/ (B+C), (A+B)/C, and (A+B)/T and SDS-sedimentation value suggested that they were more significant. The negative correlation between the ratio of (B+C)/T and SDS-sedimentation value was more significant, and the correlations between the ratio C/T and the SDS-sedimentation value were significantly negative.
Keywords:wheat  glutenin subunits  dynamic accumulation  quality
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