| 观赏海棠不同品种MYB10启动子结构分析 |
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| 引用本文： | 庞慧,田佶,姚允聪. 观赏海棠不同品种MYB10启动子结构分析[J]. 北京农学院学报, 2016, 31(4): 76-80. DOI: 10.13473/j.cnki.issn.1002-3186.2016.0416 |
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| 作者姓名： | 庞慧 田佶 姚允聪 |
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| 作者单位： | 北京农学院植物科学技术学院/北京市果树良种繁育中心/北京林果业生态环境功能提升协同创新中心,北京,102206;北京农学院植物科学技术学院/北京市果树良种繁育中心/北京林果业生态环境功能提升协同创新中心,北京,102206;北京农学院植物科学技术学院/北京市果树良种繁育中心/北京林果业生态环境功能提升协同创新中心,北京,102206 |
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| 基金项目： | 科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(2011协同创新中心)(市级)(PXM2016_014207_000038) |
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| 摘 要： | [目的]观赏海棠叶片色泽类型多样,为了研究McMYB10基因对不同叶色观赏海棠品种呈色的影响.[方法]利用PCR克隆的方法,从两个极端叶色观赏海棠品种中克隆得到两个McMYB10启动子,同时对这两个启动子在5个不同叶色类型的观赏海棠分布进行检测.[结果]McMYB10启动子在不同观赏海棠品种中存在两种不同的类型,即R1型和R6型;在McMYB10启动子中存在逆境、激素、光等多种顺式作用元件,同时在变色类观赏海棠品种中,R1和R6型启动子都存在.[结论]R1和R6型启动子在不同叶色的观赏海棠品种中,可能对叶片的呈色起较为重要的作用.
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| 关 键 词： | 观赏海棠 McMYB10 启动子 |
The study on promoter structure of MYB10 in different crabapple cultivars |
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| Abstract: | [Objective]Ornamental crabapples have diverse colors of leaves.To explore the molecular mechanism of McMYB10 affecting the coloration of leaves in different crabapple cultivars.[Methods]We cloned two McMYB10 promoters,R6 and R1,from the ever-red leaved Malus crabapple cultivar ‘Royalty’ and ever-green leaved Malus crabapple cultivar ‘Flame’ by using the method of PCR cloning,and also tested the distribution of the promoter structure in 5 crabapple cultivars.[Results] The results showed that R1 and R6 are exist in different crabapple cultivars,and the promoter of McMYB10 has many cis-elements that maybe induced by stress,hormone and light.Meanwhile,the spring-red crabapple cultivar has both R1 and R6 promoters.[Conclusion]R1 and R6 promoter play an important role in the leave coloration in crabapple. |
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| Keywords: | crabapple McMYB10 promoter |
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