Changes in platelet-activating factor receptor binding characteristics in cerebral thrombotic core and penumbra of tree shrews |
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Authors: | YANG Li-jun LI Shu-qing |
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Institution: | Department of Pathophysiology, Kunming Medical College, Kunming 650031, China |
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Abstract: | AIM: To observe the changes in platelet-activating factor (PAF) receptor binding characteristics and explore the action of PAF on formation of thrombotic core and penumbra following local cerebral ischemia. METHODS: Neuron's membrane protein was abstracted, and the local cerebral ischemia model were induced by photochemistry in tree shrews. The PAF binding sites on central neuron membrane were studied by-PAF binding assay. RESULTS: There were two different affinities of PAF receptors on tree shrew's brain cell membrane, with kD1=(3.61 ±0.72) nmol/L and kD2=(17.04±2.41) nmol/L, corresponding respectively to maximum number of binding sites: Bmax1=(1 457.94±168.01) pmol/g protein and Bmax2=(5 017.40±742.16) pmol/g protein. The binding sites decreased in ischemic core, penumbra and contralateral regions at 4,24 and 72 h after ischemia (P<0.01), with those of 24 h reaching the minimum levels. CONCLUSION: PAF receptors play an important role in cerebral ischemia, may be related to the secondary damage in ischemic penumbra, and also are molecular bases of brain injury induced by PAF. |
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Keywords: | Photochemistry Cerebral ischemia Platelet activating factor Tupaiidae |
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