Effect of 2, 3-butanedione monoxime (BDM) on myocardial protection in cold cardioplegia |
| |
Authors: | OUYANG Jing-ping LIU Yong-ming ZHENG Han-qiao WEI Lei TU Shu-zhen YANG Hai-lu CUI Ye-jian |
| |
Institution: | 1. Department of Pathophysiology, Hubei Medical University, Wuhan 430071; 2. Experimental Center, Hubei Medical University, Wuhan 430071, China |
| |
Abstract: | AIM: The effects of BDM on isolated rat heart in cold cardioplegia were studied. METHODS: Rat heart were subjected to cold cardioplegia at 4℃ for 8, 18 and 24 h.Then each heart was perfused (90 cm H2O) in Langendorff model at 37℃ for 40 min. In the high K+ group(n=24) the hearts were preserved in St.Thomas cardioplegic solution, in BDM group(n=24) hearts were preserved in K-H solution with BDM 30 mmoL/L. RESULTS: After 18 h, heart rate and the coronary flow in BDM group were significantly higher than in high K+ group(P<0.05). Activity of Na+-K+-ATPase in cell membrane and in mitochondrial membrane in the BDM group was significantly higher than high K+ group(P<0.01). After 24 h, all hearts in high K+ group were dead, but were alive in BDM group. CONCLUSION: Under given experiment conditions, BDM did enhance the tolerance to cold ischemia significantly. The results showed that BDM may become a useful agent for prolong the storage period of heart in cold cardioglegia. |
| |
Keywords: | Heart arrest induced Perfusion regional Myocardial contraction Rats |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|