Effect of Jiere Xingshen Injection on cAMP,IL-1β content in hypothalamus of ET febrile rabbits

AIM: The effects of Jiere Xingshen(JRXS) Injection on cAMP, IL-1_β content in hypothalamus (HP) of endotoxin(ET)-induced feverish rabbits were studied. METHODS: The ET-induced fever model was established in rabbits and the cAMP content in hypothalamus (HP) and csf, IL-1_β content in HP were determined by radioimmunoassay following intravenous infusion of JRXS. RESULTS: In ET group, the ΔT[(0.40±0.11)℃], TRI₁(1.78±0.79), cAMP content in HP[(2.90±0.40)nmol/g], cAMP content in csf[(0.40±0.11)nmol/L)], IL-1_β content in HP[(6.08±0.79)ng/g] were higher than that of NS and JRXS+ET group (P＜0.01). In JRXS+ET group, the ΔT[(0.10±0.10)℃], TRI₁(0.36±0.64), cAMP content in HP[(1.37±0.27)nmol/g], cAMP content in csf[(14.4±3.69)nmol/L)], IL-1_β content in HP[(2.90±0.37)ng/g] were very close to that of NS group but lower than that of the ET group (P＜0.01);The cAMP content in HP and csf, IL-1_β content in HP paralleled with the fluctuation of temperature. CONCLUTION: JRXS Injection has significant inhibitory effect on ET-induced fever by inhibiting cAMP and IL-1_β production in hypothalamus.