Effect of activation of stimulator cells on expression of CD69 by responder T cells in mixed lymphocyte reaction

AIM: To study the influence of status of stimulator cells on activation of responder T cells in mixed lymphocyte reaction (MLR), so as to provide some basis for clinical transplantation. METHODS: Stimulator cells were pretreated differently before mixed lymphocyte culture (MLC) to change their functional status, fluorescence conjugated antibodies and flow cytometry were used to detect expression of CD69 by responder T cells at several different time points.RESULTS:The expression percentages of CD69 by responder T cells in MLCa group(stimulator cells were pre-activated)were significantly higher than those in MLC group(stimulator cells were not pre-activated)at 24,48and 72 hours of culture,respectively(5.21%±0.24%vs 1.98%±0.33%,29.81%±0.85%vs 20.65%±1.00%and 39.61%±1.62%vs 13.49%±0.60%,P<0.01). CONCLUSION: Our results showed that with pre-activated stimulator cells, expression of CD69 by responder T cells could be significantly elevated in MLR.