Effects of probucol on the proliferation of rat vascular smooth muscle cells stimulated by basic fibroblast growth factor and hydrogen peroxide |
| |
Authors: | SHENG Lin PAN Qi-xing LIU Ya-jun YU Ai-qin MA Yu-lin YANG Kai |
| |
Affiliation: | 1. Department of Cardiology, the Affiliated Hospital of Shandong Medical University, Jinan 250012,China;2. Department of Cardiology, Pingdu People's Hospital, Pingdu 266700, China;3. Zichuan People's Hospital, Zichuan 255100, China |
| |
Abstract: | AIM: To investigate the effect of probucol on proliferation of rat vascular smooth muscle cells(VSMC) stimulated by basic fibroblast growth factor (bFGF) and/or hydrogen peroxide(H2O2). METHODS: Effects of probucol on VSMC proliferation and DNA synthesis stimulated by bFGF and/or H2O2 were observed by means of MTT test, cell number count and [3H]-TdR incorporation. RESULTS: ①Probucol significantly inhibited proliferation and DNA synthesis in VSMC stimulated by bFGF and/or H2O2, with dosage-dependent manner. Cell number, A value and [3H]-TdR incorporation in group probucol+bFGF and group probucol+H2O2 were reduced by 40.0%, 39.1%, 45.5% and 46.9%, 45.0%, 39.5%, respectively, compared with group bFGF and group H2O2 (P<0.05, P<0.01, respectively). ②Pretreatment of VSMC with probucol for 24 h prior to bFGF and/or H2O2 stimulation exhibited significant inhibiton of VSMC proliferation and DNA synthesis, but after prestimulation by bFGF and/or H2O2 for 24 h, probucol had no influence on VSMC proliferation and DNA synthesis (P>0.05). CONCLUSION: Probucol dramatically inhibited proliferation and DNA synthesis in VSMC stimulated by bFGF and/or H2O2, but had no inhibitory effect on the cell proliferation prestimulated by bFGF and /or H2O2. |
| |
Keywords: | Probucol Fibroblast growth factor basic Hydrogen peroxide Muscle smooth vascular Cells |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
|
点击此处可从《园艺学报》下载全文 |
|