Effects of left renal vein partial ligation on rat renal aquaporin 1 and aquaporin 2 mRNA expression

AIM:Changes of rat renal aquaporin (AQP1) and AQP2 mRNA expression after partially ligating left renal vein were observed to explore molecular mechanism of renal tubular reabsorption decrease resulted by increasing renal vein pressure.METHODS: Adult male rats were randomly divided into left renal vein partial ligation group(ie. VCG, varicocele group)and sham operation group(SOG), left renal mRNA expression was analyed Northen blot two months later. RESULTS:Intensity of left renal AQP1 mRNA expression in VCG was weaker than that in SOG, there was not any difference in left renal AQP2 mRNA expression between two groups, no hybridization signal was found in testes.CONCLUSION:Decrease of renal tubular reabsorption resulted by partially ligating renal vein might be related to decrease of AQP1 mRNA expression; "hormone escape" might occur in collecting tubules.