Effects of hypothermia on the contents of neuropetides and other active substances in rat plasma and different brain regions

AIM: To study the effects of hypothermia on the contents of AVP,Ang Ⅱ,L-EK,cAMP,Mg²⁺and Ca²⁺in rat plasma and different brain regions.METHODS: The model of experimental hypothermia rat was established and treated in groups randomly.At the end of the experiment,samples of plasma and braintissues were taken and the contents of L-EK,cAMP,AVP,Ang Ⅱ were determined by RIA methods,Mg²⁺and Ca²⁺by atom absorption methods.RESULTS: The contents of Ang Ⅱ,AVP increased significantly in the plasma of Ⅰ,Ⅲ group(P<0.01),while that of group Ⅱ decreased obviously (P<0.01);the contents of Ang Ⅱ,AVP decreased in the hypothalamus and truncus encephalicus of Ⅰ,Ⅲ group(P<0.01),and that of group Ⅱ increased (P<0.01).CONCLUSION: L-EK,cAMP,AVP,Ang Ⅱ,Mg²⁺and Ca²⁺might be involved in body temperature regulation during hypothermia.