Protective effect of cardiomyopeptidin on cultured myocardial cells injured by anoxia-reoxygenation

AIM:To study the protective effect of cardiomyopeptidin (CMP) attributed to polypeptide on cultured myocardial cells injured by anoxia-reoxygenation.METHODS:The anoxia-reoxygenation injury model were developed, anoxia for 60 min and reoxygenation for 30 min. The effect of CMP on myocardial ultrastructure was observed. Ca^2＋]_i was estimated with adherent cell analysis and sorting 570(ACAS 570) laser cytometer and measured with fluorescent dye Fura-2-AM, the lipid fluidity of cellular membrane was determined by fluorescence polarization technique. RESULTS:CMP could obviously improve the ultrastructure of myocardial cells and dose-dependently decreaseCa^2＋] _i and increase the lipid fluidity of cellular membrane, CMP also could markedly reduce the chromaticity value of pseudo-colour graphic model of Ca^2＋. CONCLUSION:Cardiomyopeptidin has an obvious protective effect on cultured myocardial cells injured by anoxia-reoxygenation, this may be related to its effect of decreasing Ca^2＋]_i and increasing lipid fluidity of cellular membrane.