The expression of hHR21sp gene in the peripheral blood cells and bone marrow cells of patients with Fanconi anemia |
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Authors: | DENG Yu-bin LI Shu-nong ZHOU Ping-kun Magali T |
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Institution: | 1. Department of Pathophysiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China;
2. Institute of Radiation Medicine, Academy of Military Medicine, Beijing 100850, China;
3. Hospital Saint-Louis, Paris 75010, France |
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Abstract: | AIM:To detect the expression of hHR21spgene in normal human peripheral blood cells and in hemapoietic cell from fanconi anemia(FA) patients bone marrow after exposure to UV and gamma-irradiation.METHODS:RNA of FA hemapoietic cell and normal peripheral blood cells were harvested after UV and gamma irradiation. hHR21sp gene expression was analyzed using RT-PCR, southern blot hybridization, phosphoimmage autoradiogram.RESULTS:The expression of hHR21sp gene in PBL varied with the time of UV gamma-irradiation and increased obviously six hours after 80 J/M2 UV-or 5 Gy gamma-irradiation. However, the expression was down-regulated in FA hemapoietic cell from FA patients indicating that hHR21sp expression is something related to the ability of cells to repair DNA double strand break.CONCLUSION:The expression of hHR21sp down-regulated in the FA syndrome and this might be involved in the pathogenesis of the FA. |
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Keywords: | Fanconis anemia Radiation ionizing Gene expression |
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