Effects of non-wounded ischemic preconditioning on ischemia-reperfusion injury in isolated rat hearts |
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Authors: | WANG Hai-hua QI Ren-bin YU Lei WANG Zhi-gang MA Ying-zhong ZHOU Zhi-yong ZUO Bao-hua |
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Affiliation: | 1. Institute of Cardiovascular Function Research, Wannan Medical College, Wuhu 241001, China;2. Grade 97 of Medical Department, Wannan Medical College, Wuhu 241001, China;3. Department of Pathophysiology, Medical College of Jinan University, Guangzhou 510632, China |
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Abstract: | AIM:To observe the protective effect of non-wounded ischemic preconditioning on ischemic/reperfusion injury in isolated rat hearts. METHODS: 25 male SD rats, weighting (250±30) g, were randomly divided into three groups: control group (C,n=8), anoxia/reoxygenation group (A,n=8) and non-wounded legs ischemic preconditioning group (N-WIP,n=9).Hearts were isolated from rats and perfused on a Langendorff apparatus with a normal Krebs-Henseleit buffer (saturation 95% O2+5% CO2) at a constant pressure (8.33 kPa) and temperature (37 ℃) in C group; Following 15 min equilibration, hearts were subjected to 15 min of global ischemia and 15 min reperfusion (37℃) in A group; Rats were subjected to non-wounded leg repeated-brief ischemic preconditioning, and then treated in procedure similar to A group in N-WIP group.The activities of superoxide dismutase (SOD) and Ca2+-Mg2+-ATPase, malondialdehyde (MDA) content of efflux from coronary vessel and myocardium, myocardium monophasic action potential and contractile force were measured before ischemia, 15 minutes after ischemia and 5, 15 minutes after reperfusion. RESULTS:Compared with A group, non-wounded legs ischemic preconditioning reduced the incidence of reperfusion arrhythmias (P<0.05), decreased the content of MDA of myocardium (P<0.01), enhanced the activities of SOD (P<0.01) and stabilized myocardial membranous potential,the activity of Ca2+-Mg2+-ATPase and contractile function. CONCLUSION:These results indicate that non-wounded leg ischemic preconditioning has a protective effect on ischemia-reperfusion injury in isolated rat hearts. The mechanism may be related to the strength of antioxidation, the stability of Ca2+-Mg2+-ATPase activity and membranous structure in myocardium. |
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Keywords: | Rats Anoxia Ischemic preconditioning Reperfusion injury Myocardium |
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