Protective effects of liposomes containing L-arginine,Se and taurine on intestinal ischemia-reperfusion injury in rats

AIM and METHODS:To study the protective effects of liposomes containing L-Arg,Se and taurine on intestinal ischemia-reperfusion injury in rats. Wistar rats were divided randomly into sham operated group,ischemia-reperfusion(I/R) group,pretreatment with liposomes group and treatment with liposomes at reperfusion group. In the experiments, superior mesenteric artery was clipped for 60 min, and then unclipped. 2 hours of reperfusion later, MDA content, T-SOD and Ca²⁺-Mg²⁺-ATPase activities in intestinal tissues were detected respectively, ultrastructure and bcl-2 expression in intestinal mucosa tissue were observed.RESULTS:MDA content in liposomes-treated group was less than I/R group (P＜0.01).The activities of T-SOD and Ca²⁺-Mg²⁺-ATPase in liposomes-treated group were higher than I/R group(P＜0.01). Bcl-2 staining was negative in I/R group, and was positive in liposomes-treated group (P＜0.01).There was no difference in above indexes between pretreatment with liposomes group and treatment with liposomes at reperfusion group(P＞0.05). CONCLUSION:Liposomes containing L-arginine, Se and taurine can protect intestine against ischemia-reperfusion injury in rats,which may be related to inhibiting lipid peroxidation, stabilizing internal circumstances and inducing bcl-2 protein expression.