Effect of compound salvae-dropping-pill on intracellular free calcium concentration of cultured rat myocardial cells in case of hypoxia and reoxygenation |
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Authors: | LI Quan-feng WANG Xiao-ming ZHU Shi-jun AI Jing GAO Huan-huan XU Chang-qing |
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Affiliation: | 1. Department of Pathophysiology,Harbin Medical University, Harbin 150086, China;2. Department of Pharmacology, Harbin Medical University, Harbin 150086, China |
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Abstract: | AIM:To examine the effect of compound salvae-dropping-pill (CSDP) on intracellular free calcium in cultured rat myocardial cells subjected to hypoxia and reoxygenation.METHODS:The Fluo- 3/AM was applied to probe intracellular calcium concentration and the fluorescent intensity was detected using laser confocal microscopy technique.RESULTS:Fluorescent intensity in hypoxia plus CSDP group was significantly lower (1 217.78±312.07) than that of hypoxia group (1 509±508.48), and the Fluorescent intensity of hypoxia/reoxygenation plus CSDP group was also markedly lower (1 567.91±577.61) than that of hypoxia/reoxygenation group (1 617.60±477.53).CONCLUSION:The cultured rat myocardial cells could be effectively protected by administration of CSDP in case of hypoxia and reoxygenation through decreasing the intracellular calcium concentration. |
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Keywords: | Drugs Chinese herbal Fluorescence Calcium Ions Myocardium Cells Anoxia |
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