Establishment of nasopharyngeal carcinoma (NPC) cell lines stable expressing NPC-derived LMP1 |
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Authors: | LAN Ke GAN Run-liang REN Cai-ping HE Zhi-wei XIE Lu YAO Kai-tai |
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Institution: | Cancer Research Institute of Hunan Medical University, Key Lab of Carcinogenesis of Chinese Ministry of Public Health, Changsha 410078, China |
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Abstract: | AIM: To establish nasopharyngeal carcinoma(NPC) cell lines stable expressing NPC-derived latent membrane protein 1(LMP1) gene.METHODS:General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed by using recombinant techniques, then transfected these vectors into a poor differentiated NPC cell line named CNE-2 ,integration and expression of N-LMP1 in CNE-2 cells were detected by PCR,RT-PCR and Western blot. RESULTS:(1) General expression vector and epithelium-specific expression vector of NPC-derived LMP1 gene were constructed successfully.(2) It showed that N-LMP1 gene expressed in CNE-2 cells correctly.CONCLUSION: The first NPC cell lines which stable express NPC-LMP1 were established. The cell lines obtained will provide important basis for exploring the role of NPC-LMP1 in nasopharynx carcinogenesis. |
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Keywords: | Membrane proteins Epstein-Barr virus Nasopharyngeal neoplasms |
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