Role of nitric oxide in ischemia/reperfusion injury and ischemic preconditioning |
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Authors: | JIAO Xiang-ying LUO Ning ZHAO Rong-rui |
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Affiliation: | 1. Department of Physiology, Shanxi Medical University, Taiyuan 030001, China;2. Shanxi Tumor Hospital, Taiyuan 030001, China |
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Abstract: | AIM: To clarify the role of nitric oxide(NO) in ischemic preconditioning(IP) and its effects on apoptosis. METHODS: Seventy-two male Wistar rats were divided into the following six groups:ischemia/reperfusion (IR) group,IP group,IR+L-arg group,IP+L-arg group,IR+L-NAME group and IP+L-NAME group,The following changes were measured:cardiac hemodynamic parameters,infarct size,PMNs counting myocardial MPO activity and TUNEL staining.RESULTS: ①L-arg significantly attenuated ischemia/reperfusion-induced heart injury,reduced PMNs infiltration and cardiomyocyte apoptosis.②L-NAME also significantly reduced infarct size,PMNs infiltration and cardiomyocyte apoptosis compared with IR group,however,L-NAME aggravated ischemia/reperfusions-induced cardiac functional injury.③L-arg or L-NAME did not significantly alter the protective effect of ischemic preconditioning. CONCLUSION: Increased production of endogenous NO before prolonged ischemic period can protect hearts and inhibit apoptosis.L-NAME can inhibit iNOS activity and ONOO- production in reperfusion period to protect heart. |
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Keywords: | Reperfusion injury Ischemic preconditioning Rats Nitric oxide Apoptosis |
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