The role of chlamydia pneumoniae in the course of transformation of C57 BL/6J mouse peritoneal macrophages into foam cells |
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Authors: | JIN Jun-fei YANG Yong-zong |
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Affiliation: | 1. Institute of Cardiovascular Disease of Hengyang Medical College, Hengang 421001, China; 2. Department of Pathophysiology, Nanjing railway Medical College, Nanjing 210009, China |
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Abstract: | AIM: In order to understand the role of chlamydia pneumoniae in the course of macrophages transformation into foam cells experiments with chlamydia pneumoniae standard strain AR-39 wese made. METHOD: C57 BL/6J Mouse peritoneal macrophages C57 BL/6J wese incubated 24 h, and they were divided into 6 groups to be incubated continually: A1~DMEM; A2~DMEM+10 IFUs/L AR-39; B1~DMEM+10mg/L LDL; B2~DMEM+10mg/L LDL+10 IFUs/L AR-39; C1~DMEM+10mg/L OxLDL;C2~DMEM+10mg/L OxLDL+10 IFUs/L AR-39. 72 h later, morphological changes of the cells were observed and of intracellular cholesterol of content was detected. RESULTS: 1、Morphological studies: there were no lipid particles in A1, A2 and B1 groups, but the lipid particles could be found in B2、C1 and C2 group, Among six groups, the most lipid particles were seen in C2 groups. 2、Biochemical detection:The ratio of cholesterol ester to total cholesterol was much higher than 50% in B2、C1 and C2 groups, but was less than 50% in A1、A2 and B1 groups. CONCLUSION: Chlamydia pneumoniae may have played a role in promoting C57 BL/6J mouse peritoneal macrophages into foam cells. |
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Keywords: | Chlamydia pneumoniae Macrophages Foam cells Atherosclerosis |
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