Expression of PLIN3 in lung adenocarcinoma and its correlation with prognosis

AIM To explore the expression of perilipin 3（PLIN3） in lung adenocarcinoma and the relationship between the prognosis of patients and the invasiveness of lung adenocarcinoma cells. METHODS HPA database was used to predict the genes related to poor prognosis of lung cancer and PLIN3 was selected as the research object.HPA database was used to analyze the correlation between PLIN3 and survival rate of lung cancer and lung adenocarcinoma.GEPIA database was used to further verify the correlation between the expression difference of PLIN3 and the survival rate of lung adenocarcinoma patients. The expression of PLIN3 in lung adenocarcinoma was further analyzed by using Ualcan database.Western blot was used to detect the expression of PLIN3 in lung adenocarcinoma cells.siPLIN3 plasmid was constructured and Transwell assay was used to detect the invasion ability of A549 cells after transfection. RESULTS PLIN3 was significantly related to the survival rate of the patients with lung adenocarcinoma and it was over-expressed in lung adenocarcinoma tissues.The expression of PLIN3 was closely related to the stages of cancer and the grades of lymph node metastasis of lung adenocarcinoma.PLIN3 was over-expressed in lung adenocarcinoma cells. The number of the A549 cells passing through Transwell in knock-down group was significantly lower than that in control group. CONCLUSION PLIN3 is highly expressed in lung adenocarcinoma. The expression level of PLIN3 is related to the prognosis of lung adenocarcinoma patients.Knock-down of PLIN3 inhibits the invasion ability of lung adenocarcinoma cells in vitro.