Effects of norepinephrine preconditioning and ischemic preconditioning on apoptosis and Bcl-2, Bax expression in rat myocardial cells during myocardial ischemic reperfusion |
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Authors: | CHENG Fang-zhou TANG Guo-hua LI Geng-shan YU Xi-qiu LI Yuan-zhong HE Xin-hua BAO Cui-yu ZHOU Yun |
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Institution: | 1. Department of Cardiology, People's Hospital, Wuhan University, Wuhan 430060, China;
2. Department of Internal Medicine, First Affiliated Hospital, Xianning Medical College, Xianning 437100, China;
3. Department of Cardiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China |
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Abstract: | AIM: To study the effects of norepinephrine preconditioning(NE-P) and ischemic preconditioning (IP)on apoptosis and Bcl-2, Bax expression in rat myocardial cells in myocardial ischemic reperfusion (I/R). METHODS: The model of rat ischemic-reperfusion was used to conduct NE-preconditioning. Apoptotic myocytes were detected with TUNEL. Bcl-2, Bax expression were detected with immunohistochemistry. RESULTS: The rate of apoptosis cells in I/R group was higher, the rate of apoptosis cells in NE-P group and IP was lower significantly than that in I/R group(P<0.01). The expression of Bcl-2 in I/R group was lower, but the expression of Bax was higher, the expression of Bcl-2 in NE-P group was higher significantly than that in I/R group(P<0.01), the expression of Bax in NE-P group was lower than that in I/R group(P<0.01). There was no significantly difference between NE-P and IP group in the above parameters (P>0.05). CONCLUSION: NE-P reduced myocyte apoptosis by I/R in rats; The expression of Bcl-2 ,Bax genes played an important role in myocardial apoptosis. |
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Keywords: | Ischemic preconditioning myocardial Reperfusion Apoptosis Rats |
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