Effect of epidermal growth factor receptor antibody on human colon carcinoma

AIM: To observe the effect of monoclonal antibody of epidermal growth factor receptor(EGFR) on human colon carcinoma cell lines. METHODS: Cell counting, growth curve measurement and MTT method were applied in this study to examine the proliferation of cultured cells in vitro when different dosage of EGFR McAb is used to treat LST174 colon carcinoma cell lines. RESULT: The proliferation of cultured human colon carcinoma cells could be significantly inhibited in a dose dependent manner by EGFR antibody Compared with the control group, the cell number was decreased by 61.3% and 33.8% respectively when treated with 0.625 mL/L or 2.5 mL/L of EGFR McAb CONCLUSION: EGFR McAb can inhibit cell growth of human colon carcinoma LST174.