Effect of PKC activition on cardiac myocyte apoptosis and Bcl- 2 expression during myocardial ischemia/reperfusion in rats |
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| Authors: | LIU Xing-de CHEN Yun-zhen |
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| Institution: | 1. Department of Cardiology, Affiliated Hospital of Guiyang Medical College , Guiyang 550004, China;
2. Department of Cardioloy, First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400015, China |
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| Abstract: | AIM: To explore the effect of PKC activition on cardiac myocyte apoptosis and expression of bcl- 2 during myocardial ischemia/reperfusion(I/R) in rats. METHODS: TUNEL,immunohistochemistry and in situ hybridization were used. RESULTS: The TUNEL data showed that the numbers of positive cardiac myocyte nucleus and the percentage of positive cardiac myocyte nucleus in PMA+IR3 h group decreased significantly(P＜0.05,P＜0.01), compared to those in IR3h group. The number of Bcl-2 protein positive cardiomyocytes and the percentage of Bcl-2 protein positive cardiomyocytes in PMA+IR3h group were higher than those in IR3h group (P＜0.01) bcl- 2 mRNA expression showed the same changes in PMA+IR0h group compared to IR1h group.CONCL USIONS:Activation of PKC decreased cardiomyocyte death during I/R.Upregulation of bcl-2 gene expression in cardiomyocytes during I/R may be one of the mechanisms of decreasing cardiomyocyte death by PCK activating during I/R. |
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| Keywords: | Myocardial reperfusion Cell death Gene expression Protein kinase C Rats |
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