Effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata and the ascites drainage

AIM: To further study the effect of Ershen Zeshu Mixture, a Chinese medicine, on the peritoneal lymphatic stomata and the ascites drainage in experimental mice model of CCl₄-induced fibrosis.By the application of NO supplier (sodium nitroprusside,SNP) and NO suppressor (N^G-mononethyl-L-arginine,L-NMMA),the effect of NO on the peritoneal lymphatic stomata and the ascites drainage was studied to clarify the relationship between Ershen Zeshu Mixture and NO in the regulation of the peritoneal lymphatic stomata.METHODS: The mouse model of fibrosis was established with the application of intragastric installations of carbon tetrachloride one time every three days;Scanning electron microscope and computer image processing were used to detect the girth,area and distribution density of the peritoneal lymphatic stomata;the concentration of urinary ions was measured in the experiment.RESULTS: ①Compared to NS group and model group,Ershen Zeshu Mixture could significantly increase the girth and the area of the peritoneal lymphatic stomata,promote its distribution density (P <0.01) and enhance the drainage of urinary ions such as sodium,potassium and chlorine(P<0.05 or P <0.01).②The effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata was altered by adding NO supplier or NOS suppressor to the peritoneal cavity,that is,NO supplier can enhance the regulation effect of Ershen Zeshu Mixture on the peritoneal lymphatic stomata,but NO suppressor can antagonize the Ershen Zeshu Mixture's effect.CONCLUSIONS: ①These observations indicated that Ershen Zeshu Mixture might regulate the peritoneal lymphatic stomata to drainage materials in the peritoneal cavity.②The effect of Ershen Zeshu Mixture on lymphatic stomata and ascites drainage maybe have relationship with endogenous NO.