Role of nuclear factor κB on the expression of interleukin-6 in mouse mesangial cells induced by interleukin-1β |
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Authors: | XU Han-shi YE Ren-gao YANG Qiong-qiong SUN Lin YANG Nian-sheng LI You-ji ZENG Li-xia |
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Affiliation: | Department of Nephrology, The First Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China |
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Abstract: | AIM:To investigate the regulatory role of nuclear factor κB (NF-κB) in the expression of interleukin-6 in mesangial cells (MC) induced by interleukin-1β.METHODS:Activation of NF-κB was measured by electrophoresis mobility shift assay (EMSA). RT/PCR and ELISA were used to detect IL-6 mRNA expression and IL-6 production, respectively.RESULTS:rhIL-1β could rapidly stimulate the activation of NF-κB in MC, and increase the expression of IL-6 mRNA and protein. PDTC, one of the inhibitor of NF-κB, could inhibit the expression of IL-6 in mRNA and protein in MC stimulated by rhIL-1β.CONCLUSION:IL-6 expression induced by IL-1β may be regulated by NF-κB in MC, NF-κB may modulate the immune-inflammatory reaction in glomerular disease. |
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Keywords: | Interleukin- Interleukin-6 NF-Kappa B Kidney glomerulus Mice |
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