Brain damage of cerebral ischemia/reperfusion and expression of TGF-β1 mRNA in diabetic rats |
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Authors: | WANG Gui-xia LI Guang-ren OU YANG Yi-bing YANG Tong-shu WANG Shao |
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Institution: | 1. The First Clinical Hospital,Nor man Bethune Medical University,Changchun 130021,China;
2. Institute of Preclinic Sciences,Nor man Bethune Medical University,Changchun 130021,China;
3. Basic School of Medical Sciences,Nor man Bethune Medical University,Changchun 130021,China |
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Abstract: | AIM:To observe the difference of cerebral inj ury following ischemia/reperfusion and mR-NA expression of TGF-β1 between diabetic and non-diabetic rats.METHODS:At first,Wistar rats weredivided i nto two groups,non-diabetes and diabetes,and then two groups followed by sham,middle cerebralartery occlusion(MCAO) 2h and reperfusion 24 h after MCAO 2 h respectively.TGF-β1 mRNA expressionwas measured by semi-quantitative reverse transcri ption polymerase chain reaction(RT-PCR);Cerebraldamage was eval uated by histopathology.RESULTS:In the same condition of ischemia or ischemia/reperfu-sion,inj uried area enlarged in DMgroups;The expressionlevel of TGF-β1 mRNA increased at the ti me of 2h after MCAOi n non-diabetic group and diabetic group,especialy significantly in non-diabetic group withMCAO 2 h,and decreased at the time of reperfusion 24 h after MCAO 2 h,but still higher than that in theshamgroup.CONCLUSION:Diabetes mellitus exacerbated brain lesion following ischemia/repefusion,in-creased TGF-β1 mRNA expresion after MCAO may be an anti-injury reaction,and anti-injury abilityis de-creased under diabetic condition. |
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Keywords: | Diabetes mellitus Mesencephalon Arteries Infarction Transforming growth factor beta RNA messenger |
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