辽东山区两种针叶人工林土壤真菌群落结构特征 |
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引用本文: | 邓娇娇,周永斌,殷有,白雪娇,高慧淋,朱文旭. 辽东山区两种针叶人工林土壤真菌群落结构特征[J]. 北京林业大学学报, 2019, 41(9): 130-138. DOI: 10.13332/j.1000-1522.20180147 |
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作者姓名: | 邓娇娇 周永斌 殷有 白雪娇 高慧淋 朱文旭 |
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作者单位: | 1.沈阳农业大学林学院,辽宁 沈阳 110161 |
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基金项目: | 国家科技支撑(2015BAD07B010303),国家重点研发计划项目子课题(2017YFC050410501),辽宁省农业领域青年科技创新人才培养计划项目(2015047),辽宁省宜林地营林决策平台构建及示范项目(201304216) |
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摘 要: | 目的为探讨辽东山区两种典型针叶人工林土壤真菌群落多样性及结构特征,揭示真菌群落结构与树种、土壤环境因子的相关性。方法本研究以辽东山区白石砬子自然保护区内落叶松(LGe)、红松(PKe)人工林和辽宁省森林经营研究所实验林场落叶松(LGd)、红松(PKd)为研究对象,采用Illunima Miseq高通量测序技术和OTU分析法比较不同针叶人工林土壤真菌群落结构差异,分析优势菌群与土壤理化性质的相关性。结果(1)与红松人工林相比,落叶松人工林有助于提高土壤全碳、全氮和速效氮的含量。(2)该区落叶松和红松人工林土壤共检测到9个土壤真菌门,优势菌门为担子菌门、子囊菌门、接合菌门和隐真菌门。(3)LGe和PKe,LGd和PKd土壤真菌的多样性和丰富度指数存在差异,但都不显著。(4)Venn和Heatmap表明落叶松和红松人工林土壤真菌群落组成和相对丰度存在差异,LGe和PKe间的差异较LGd和PKd间的差异小。(5)RDA分析与Pearson相关性分析表明,土壤pH、土壤全碳、速效氮、土壤碳氮比是该区针叶人工林土壤真菌群落结构变化的关键影响因素。结论土壤真菌群落结构、多样性指数在不同树种间存在一定差异,LGe和PKe土壤有机质和真菌群落结构多样性差异较小,表现趋同性,LGd和PKd差异较大。
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关 键 词: | 辽东地区 针叶林 高通量测序 真菌群落结构 |
收稿时间: | 2018-04-27 |
Characteristics of soil fungal community structure at two coniferous plantations in mountainous region of eastern Liaoning Province,northeastern China |
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Affiliation: | 1.College of Foresty, Shenyang Agricultural University, Shenyang 110161, Liaoning, China2.Research Station of Liaohe-River Plain Forest Ecosystem, Chinese Forest Ecosystem Research Network, Tieling 112500, Liaoning, China |
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Abstract: | ObjectiveThis paper aims understand the diversity and community structure of soil fungi in different coniferous plantations in mountainous region of Eastern Liaoning Province, China, and reveal the relationship between fungal community diversity and structure and forest types as well as soil environment factors. MethodEnvironmental genomics approaches, including Illumina MiSeq high throughput sequencing and OTU analysis, were used to assess the composition and structure of fungal community in Larix gmelinii (LGe) and Pinus koraiensis (PKe) in Beshila Nature Reserve, Larix gmelinii (LGd) and Pinus koraiensis (PKd) in the experimental forest farm of Liaoning Institute of Forest Management in Montane Region of Eastern Liaoning Province. Result(1) Compared with Pinus koraiensis, Larix gmelinii can improve the contents of total carbon, total nitrogen and available nitrogen in soil. (2) The results showed that 9 different fungal phyla were identified in the soil of Larix gmelinii and Pinus koraiensis plants. The dominant fungal phylum groups were Basidiomycota, Ascomycota, Zygomycota and Rozellomycota. (3) Structurally, there were no significant difference in diversity indices and richness indices between LGe and PKe, so were LGd and PKd. (4) The Venn diagram and heatmap showed that the fungal community composition and relative abundance of Larix gmelinii and Pinus koraiensis plants were different, and the difference between LGe and PKe was smaller than that between LGd and PKd. (5) Redundant analysis and correlation analysis indicated that soil pH, TC, AN and C/N were the main factors which affected the community structure of coniferous forests in this area. Conclusion This work showed that there were some differences in soil fungal community structure and diversity index among different forest types. The soil organic matter and fungal communities of the LGe and PKe showed small discrepancy compared with LGd and PKd, and planting Larix gmelinii helps improve soil fertility. |
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