Effects of LPS on expressions of ET-1, eNOS and iNOS mRNA in human umbilical vein endothelial cells |
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Authors: | LI Peng LI Shan-shan HUANG Qi-fu |
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Institution: | Pathology Department, Beijing University of Chinese Medicine, Beijing 100029, China |
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Abstract: | AIM: To observe the direct effect of LPS on expressions of ET-1, eNOS, and iNOS mRNA in human umbilical vein endothelial cells, and further research the molecular mechanism of effect of LPS on production of ET-1 and NO. METHODS:The third passage of cultured human umbilical vein endothelial cells was incubated with low concentration (100 μg/L) of LPS for 6 h. Total RNA was extracted. The expressions of ET-1, eNOS, and iNOS mRNA were analyzed by semi-quantitative RT-PCR method. RESULTS: ET-1 mRNA experession increased significantly, while expression of eNOS mRNA decreased significantly, and there was no significant change in expression of iNOS mRNA. CONCLUSION: In human umbilical vein endothelial cells, low concentration of LPS enhanced the expression of ET-1 mRNA, inhibited the expression of eNOS mRNA, and had no significant effect on the expression of iNOS mRNA. |
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Keywords: | Lipopolysaccharides Umbilical veins Endothelium Endothelin-1 Nitric oxide synthase |
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