Inhibitory effect of basic fibroblast growth factor on apoptosis induced by β-amyloid in PC12 cells |
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| Authors: | ZHU Da-ming SONG Zhao-lei SUN Fen-yong YANG Yuan DUAN Rui LIN Jian HONG An |
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| Affiliation: | 1. Bioengineering Institute of Jinan University, Guangzhou 510632, China;2. Lab of Light Transmission Optics, South China Normal University, Guangzhou 510631, China |
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| Abstract: | AIM:To clarify the effect of bFGF on the neurotoxity of Aβ25-35 in PC12 cells and its potential application in the treatment of Alzheimer's disease. METHODS:Giema's, PI stainning, DNA agarose gel electrophoresis, Western blot and FCM were used to detect the morphological and biochemical changes of cultured PC12 cells treated with Aβ25-35 and bFGF+ Aβ25-35, and the expression of apoptosis-related gene bcl-2, bax. RESULTS:Morphological and biochemical characteristics of apoptosis, such as internuclear DNA fragmentation, compaction of nuclear chromatin, membrane blobbing, formation of apoptotic bodies, were observed in PC12 cells treated with Aβ25-35. However, in PC12 cells treated with bFGF+ Aβ25-35 , the above changes were significantly reversed, the expression of Bcl-2 was up-regulated while that of Bax was down-regulated. CONCLUSION:bFGF can inhibit the neurotoxity of Aβ25-35 to neurons by regulating the expression of the apoptosis-related gene Bcl-2 and Bax. |
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| Keywords: | Fibroblast growth factor basic Apoptosis Alzheimer's disease Amyloid beta-protein |
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