Whole cell screening of phage-display peptide library for mimicry peptides of glioma SWO-38 |
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Authors: | GAO Shuang-rong ZHONG Xue-yun YAN Yan-ling ZHONG Zhen-yu QIN Yan-fang SUN Yan-hua WANG Bing YU Li-na ZHONG Ying |
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Institution: | Department of Pathology, Medical College of Jinan University, Guangzhou 510632, China |
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Abstract: | AIM and METHODS:The Ph.D.-7 phage display library was used to isolate peptides specific for glioma SWO-38 cell by whole cell screening.Moreover,binding efficiency analysis was carried out to test the binding specificity of the clones obtained.RESULTS:After three rounds of biopanning,a high concentration of phage clones was obtained and two of them were found to be highly specific to glioma SWO-38.CONCLUSION:Highly specific clones against neurtral glioma cel s can be obtained from a phage display library by simple procedures. |
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Keywords: | Phage Glioma Clone cells |
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