Study on biological activity of thrombopoietinⅡ in vivo |
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| Authors: | ZHONG Li-ye CHEN Yun-xian ZHONG Xue-yun LIU Tian-hao OU Rui-ming ZHAO Hong-yun SU Wei-wei LIN Wei DU Xin XU Pei-lin |
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| Affiliation: | 1. The Department of Hematology, The First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080,China;2. Jinan University Medical College,Guangzhou 510632. China;3. he Department of Hematology, Guangdong People’s Hospital,Guangzhou 510080, China;4. The School of Life Sciences,Zhongshan University,Guangzhou 510275,China |
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| Abstract: | AIM: To investigate the biological activity of thrombopoietin Ⅱ(TPOⅡ) in vivo, which consists of two new kinds of ligand binding with thrombopoietin receptor. METHODS: Purified ligandⅠof TPOⅡ, artificial compound ligandⅡ of TPOⅡand rhTPO were injected into purebred Babl/c mice respectively in 7 days by intraperitoneal injection once for a day. Then the biological activity of TPOⅡ was analyzed by measuring peripheral platelet counts by the end of the seventh day. RESULTS: On the seventh day, the platelet counts of mice treated by ligandⅠof TPOⅡ were higher than that in the negative control group(P＜0.05), while not significantly different from the platelet counts of mice treated by rhTPO(P＞0.05). On the fourteenth day, the platelet counts increased in two all experimental groups of TPOⅡcompared with negative control group(P＜0.01), while not significantly different from the platelet counts of mice treated by rhTPO too(P＞0.05). Moreover the platelet counts of mice in two experimental groups of TPOⅡ and the positive group showed increase with experimental days. CONCLUSION: The purified ligandⅠof TPOⅡ had obvious activity in increasing platelet production, which is not different from the effect of rhTPO. |
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| Keywords: | Thrombopoietin Blood platelets Mice |
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